Phospho-AKT1 (Ser473) Rabbit anti-Human, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137046
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.The serine threonine protein kinase encoded by the AKT-1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT-1 and the related AKT-2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT-1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene). The antibody labels activated AKT-1 that is phosphorylated at 473 serine residue.
|PBS with 1% BSA and 0.099% sodium azide; pH 7.6|
|AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, PKB alpha, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Synthetic peptide derived from the region of human AKT-1 that contains phosphorylated site of serine 473.|
|Antigen affinity chromatography|
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