anti-phospho-Akt1/PKBα (Ser473), Clone: 11E6, Millipore™ (Upstate™)

Manufacturer: EMD Millipore 05669DEL

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Catalog No. 50-171-757


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    Antigen phospho-Akt1/PKBα (Ser473)
    Conjugate Unlabeled
    Cross Reactivity Canine,Human,Mouse,Rat
    Format Semi-purified
    Host Species Mouse
    Immunogen Peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.
    Isotype IgG1 κ
    Quantity 100μg
    Regulatory Status RUO
    Research Discipline Cell Signaling
    Primary or Secondary Primary
    Monoclonal or Polyclonal Monoclonal

    The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. GenBank Number: NM_005163. MW: 60kDa.

    ELISA, Western Blotting

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