Phospho-BAD (Ser99) Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA512553
This antibody is predicted to react with mouse and rat based on sequence homology.Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
|PBS with 0.09% sodium azide|
|Bcl2-antagonist of cell death; BAD; Bcl-2 binding component 6; Bcl-XL/Bcl-2-associated death promoter; Bcl-2-like 8 protein; BAD; BBC6; BCL2L8;|
|-20° C, Avoid Freeze/Thaw Cycles|
|Dot Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry|
|KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad|
|Protein A, Antigen Affinity Chromatography|
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