Phospho-CREB (Ser133) Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1851B
PA1-851B detects the phosphorylated form of cyclic-AMP response element binding protein (CREB) from rat and human cells. This antibody does not detect the unphosphorylated form of CREB. PA1-851B has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~43 kDa protein representing phospho-CREB from forskolin treated PC12 cell lysate. The PA1-851B immunogen is a synthetic phospeptide corresponding to residues K(123) R R E I L S R R P S(p) Y R K(136) of human CREB. This sequence is completely conserved between human, bovine, canine, mouse, pig, sheep, and rat CREB protein. The PA1-851B immunizing peptide (Cat. No. PEP-143) is available for use in neutralization and control experiments.CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.
|Synthetic phosphopeptide corresponding to residues K(123) R R E I L S R R P S(p) Y R K(136) of human CREB.|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Phospho-Cyclic AMP Response Element Binding Protein|
|-20° C, Avoid Freeze/Thaw Cycles|
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