Phospho-ELK1 (Ser383) Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA14625
This antibody is predicted to react with canine, mouse, non-human primate and zebrafish based on 100% sequence homology. This antibody is specific for ~46 kDa Elk-1 phosphorylated at Ser383 in Western blots using phospho and dephospho Elk-1 recombit protein. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.
|0.01M HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5|
|ETS domain protein Elk1, Oncogene Elk1|
|-20° C, Avoid Freeze/Thaw Cycles|
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 of human ELK1 conjugated to KLH|
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