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Phospho-CRYAB (Ser45) Rabbit anti-Bovine, Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1011

Catalog No. PA1011

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PA1-011 detects phospho-alpha-B crystallin (Ser45) from bovine, human, mouse and rat samples. This antibody does not detect the unphosphorylated form of the protein. PA1-011 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~20 kDa protein representing recombit bovine phospho-alpha-B crystallin (Ser45). Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining. The PA1-011 immunogen is a synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens.   Alpha-Crystallin comprises 40% of total lens protein composition.  In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation.  It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintece. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly



Phospho-CRYAB (Ser45)
PBS with 30mg/mL BSA and 0.05% sodium azide
P02510, P02511, P23927, P23928
100 μg
-20° C, Avoid Freeze/Thaw Cycles
12955, 1410, 25420, 281719
Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.
Antigen affinity chromatography
Bovine, Human, Mouse, Rat
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