Phospho-GluR1 (Ser831) Rabbit anti-Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA184839
Predicted to react with chicken, dog, human and mouse based on sequence homology. For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM NaF should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. PA1-84839 detects a band of ~100kDa in rat brain lysates. Rabbit anti Rat GluR1 (pSer831) antibody recognizes glutamate receptor 1 (GluR1), also known as AMPA-selective glutamate receptor 1, GluR-A, GluR-K1 and glutamate receptor ionotropic , AMPA 1 (GluA1), when phosphorylated at serine 831.GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
|0.01M HEPES with 0.01% BSA, 50% glycerol and 0.09% sodium azide; pH 7.5|
|GLUH1, GLUR1, GLURA, HBGR1, MGC133252|
|4°C or -20°C if preferred|
|Synthetic phosphopeptide corresponding to amino acid sequence within GluR1 which includes phosphorylated Ser831|