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Phospho-HSP27 (Ser15) Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1016

Catalog No. PA1016

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PA1-016 detects phosphorylated heat shock protein 27 (hsp27) from rat and human tissue tissues. PA1-016 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects a ~27 kDa protein which corresponds to phospho-hsp27 (Ser15) from HeLa cells. For immunolocalization, Cat. No. PA1-018 is recommended. The PA1-016 immunogen is a synthetic phosphopeptide corresponding to residues L(10) L R G P (pS) W D P F R C(21) of human HSP27. The immunizing peptide is 81% conserved in mice, rats, and hamsters. The phosphorylated peptide (Cat. No. PEP-187) and non-phosphorylated peptide (Cat. No. PEP-240) are available for use in neutralization and control experiments.

HSP27 also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP ft.s and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that HSP27 may be a useful marker in classifying various hormone sensitive tumors.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly



Phospho-HSP27 (Ser15)
Synthetic phosphopeptide corresponding to residues L(10) L R G P (pS) W D P F R C(21) of human HSP27.
Antigen affinity chromatography
Human, Rat
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
P04792, P42930
Phospho-HSP 27; Heat shock protein beta-1; HspB1; HSP25; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein; HSPB1; HSP27
100 μg
-20° C, Avoid Freeze/Thaw Cycles
24471, 3315
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