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Phospho-IRS1 (Ser636) Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA11055

Catalog No. PA11055

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PA1-1055 detects phospho-IRS-1 (Ser636) in mouse and human cells. PA1-1055 has been successfully used in WB procedures. By Western blot, this antibody detects a ~170 kDa protein representing phospho-IRS-1 (Ser636) from 3T3 L1 cells treated with insulin for 10 minutes. The PA1-1055 immunogen is a synthetic phosphopeptide corrsponding to residues D(631) Y M P M (pS) P K S V S A P Q Q I(646) of human IRS-1. The peptide sequence is 100% conserved in rats, and mice. This peptide (Cat. No. PEP-185) is available for use in neutralization and control experiments.

Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm’s tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option.To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly



Phospho-IRS1 (Ser636)
Synthetic phosphopeptide corrsponding to residues D(631) Y M P M (pS) P K S V S A P Q Q I(646) of human IRS-1.
Antigen affinity chromatography
Human, Mouse
Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
P35568, P35569
Phospho-Insulin Receptor Substrate-1
100 μg
-20° C, Avoid Freeze/Thaw Cycles
16367, 3667
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