Phospho-ERK1/ERK2 (Thr202, Tyr204) Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA14607
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.In Western blot, this antibody detects an ~42-44kDa protein representing Phospho-Thr202/Thr204 ERK/MAPK.Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay.
|Phospho-ERK1/ERK2 (Thr202, Tyr204)|
|0.01M HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5|
|P27361, P28482, P21708, P63086|
|Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2 , MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3, p44, p42|
|-20° C, Avoid Freeze/Thaw Cycles|
|116590, 50689, 5594, 5595|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat MAPK1/2 conjugated to KLH|
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