Phospho-NMDAR2B (Tyr1472) Rabbit anti-Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA186558
PA1-86558 specifically recognizes the 180 kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor when phosphorylated at Tyr1472. Predicted to react with bovine, chicken, dog, human, monkey, mouse and zebrafish based on sequence homology. For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. In Western blot, this antibody detects a band at ~180 kDa in rat brain hippocampal lysate. In Western blot, this antibody also labels proteins of 65kDa and 115kDa.NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal domit 6-related mental retardation.
|0.01M HEPES with 0.01% BSA, 50% glycerol and 0.09% sodium azide; pH 7.5|
|Synthetic phosphopeptide corresponding to an amino acid sequence within the NR2B subunit of the NMDA receptor, which includes phosphorylated Tyr1472.|
|4°C or -20°C if preferred|
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