Phospho-p38 MAPK alpha (Thr180, Tyr182), Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA112741

Catalog No. 01-674-866

Description

p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation and other signals. p38 MAPK is relatively inactive in the non-phosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, a, b, g and d, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor-a, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 and -6 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

In Western blot, immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide; immunolabeling can be completely eliminated by treatment with lambda-phosphatase. A suggested positive control for this product is C-6 glioma cell lysate.

Specifications

• Antigen: Phospho-p38 MAPK alpha (Thr180, Tyr182)
• Classification: Polyclonal
• Formulation: HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5
• Gene Accession No.: Q16539, P47811, P70618
• Gene Symbols: MAPK14
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding phospho Thr180/Tyr182 of p38
• Quantity: 10 Miniblots
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Dot Blot, Western Blot
• Conjugate: Unconjugated
• Gene: MAPK14
• Gene Alias: Map kinase
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1432, 26416, 81649
• Content And Storage: Store at -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid