Phospho-p38 MAPK alpha (Thr180, Tyr182), Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA112741
p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation and other signals. p38 MAPK is relatively inactive in the non-phosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, a, b, g and d, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor-a, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 and -6 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.In Western blot, immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide; immunolabeling can be completely eliminated by treatment with lambda-phosphatase. A suggested positive control for this product is C-6 glioma cell lysate.
|Phospho-p38 MAPK alpha (Thr180, Tyr182)|
|HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5|
|Q16539, P47811, P70618|
|-20° C, Avoid Freeze/Thaw Cycles, Avoid Freeze/Thaw Cycles|
|1432, 26416, 81649|
|Dot Blot, Western Blot|
|Synthetic phosphopeptide corresponding to amino acid residues surrounding phospho Thr180/Tyr182 of p38|
|Human, Murine, Rat|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok