Phospho-p38 MAPK alpha (Thr180, Tyr182), Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA112741
p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation and other signals. p38 MAPK is relatively inactive in the non-phosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, a, b, g and d, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor-a, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 and -6 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.In Western blot, immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide; immunolabeling can be completely eliminated by treatment with lambda-phosphatase. A suggested positive control for this product is C-6 glioma cell lysate.
|Phospho-p38 MAPK alpha (Thr180, Tyr182)|
|HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5|
|Q16539, P47811, P70618|
|-20° C, Avoid Freeze/Thaw Cycles|
|1432, 26416, 81649|
|Dot Blot, Western Blot|
|Synthetic phosphopeptide corresponding to amino acid residues surrounding phospho Thr180/Tyr182 of p38|
|Human, Murine, Rat|
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