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Phospho-c-Raf (Ser301) Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA14639

Catalog No. PA14639

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This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate and Xenopus based on 100% sequence homology. This antibody is specific for the ~74 kDa Raf-1 protein phosphorylated at Ser301. Immunolabeling can be blocked by lambda-phosphatase treatment.

The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chon et al, 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al, 2005) with two sites participating Ser 301 and Ser 642.


Phospho-c-Raf (Ser301)
0.01M HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5
P04049, P11345, Q99N57
Craf 1 transforming gene, Craf1 transforming gene, Murine sarcoma 3611 oncogene 1, Raf proto oncogene serine/threonine protein kinase, vraf1 murine le
100 μL
-20° C, Avoid Freeze/Thaw Cycles
110157, 24703, 5894
Western Blot
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 of rat RAF1 conjugated to KLH
Affinity chromatography
Human, Rat
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