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Phospho-RSK1 (Ser221), Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA124769

Catalog No. PIPA124769

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.



This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

PA1-24769 detects RSK1 p90 (phospho S221) from human samples. PA1-24769 is expected to cross react with mouse (100% conserved) and rat (100% conserved) due to sequence homology. Cross reactivity with RSK2 is expected (100% conserved). Cross reactivity with RSK3 (80% homology) and RSK4 (90% homology) is possible but has not been tested. Not yet tested in other species.PA1-24769 has been successfully used in Western blot procedures. A431 and NIH3T3 cells treated with PMA can be used as a positive control for this antibody. The PA1-24769 immunogen is a synthetic phosphopeptide corresponding to a portion of human RSK1 containing serine 221.Store at 4C short term. For extended storage, aliquot and store at -20C or below, avoiding freeze-thaw cycles.


Phospho-RSK1 p90 pSer221
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide; pH 7.3
Q15418, P18653, Q63531
RP11-492M19.2, HU-1, MAPKAPK1A, RSK, RSK1
Store at -20°C
Western Blot
Synthetic phosphopeptide corresponding to a portion of human RSK1 containing serine 221.
Antigen affinity chromatography
Human, Murine, Rat
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