Phospho-AANAT (Ser206) Rabbit anti-Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1763
Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.PA1-763 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues.PA1-763 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23kDa protein representing phosphorylated AA-NAT from rat pineal gland whole protein extract. PA1-763 immunizing phosphopeptide (Cat. # PEP-066) corresponds to carboxy-terminal amino acid residues 198-208 of rat AA-NAT. Total anti-carboxy-terminal AA-NAT-specific IgG (Cat. # PA1-762) was affinity purified on resin-coupled non-phosphopeptide (Cat. # PEP-065). Phospho-specific carboxy-terminal IgG (Cat. # PA1-763) was subsequently isolated on resin-coupled phosphopeptide (Cat. # PEP-066). This sequence is completely conserved between rat and mouse AA-NAT protein.
|Synthetic peptide: C(195) H T F L R R N S(p) G C(205)|
|Antigen affinity chromatography|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|-20° C, Avoid Freeze/Thaw Cycles|
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