Phospho-AANAT (Thr29) Rabbit anti-Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1761
PA1-761 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues. PA1-761 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract. PA1-761 immunizing phosphopeptide (Cat. No. PEP-064) corresponds to amino-terminal amino acid residues 24-33 of mouse AA-NAT. Total anti-amino-terminal AA-NAT-specific IgG (Cat. No. PA1-760) was affinity purified on resin-coupled non-phosphopeptide (Cat. No. PEP-063). Phospho-specific amino-terminal IgG (Cat. No. PA1-761) was subsequently isolated on resin-coupled phosphopeptide (Cat. No. PEP-064). This sequence is completely conserved between mouse, rat, and human AA-NAT protein.Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
|Synthetic Peptide: C(24) Q R R H T(p) L P A S(33)|
|Antigen affinity chromatography|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|-20° C, Avoid Freeze/Thaw Cycles|
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