PICK1 Rabbit anti-Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1073
PA1-073 detects protein interacting with protein kinase C (PICK1) from mouse cells. PA1-073 has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~52 kDa protein representing PICK1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7. The PA1-073 immunogen is a synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1. The PA1-073 immunizing peptide (Cat. No. PEP-133) is available for use in neutralization and control experiments.PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.
Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
|Synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1.|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Protein Interacting with Protein Kinase C, PRKCA-binding protein, Protein kinase C-alpha-binding protein, Protein interacting with C kinase 1|
|-20° C, Avoid Freeze/Thaw Cycles|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok