PKR Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA520284
A suggested positive control is Caco-2 cell lysate. PA5-20284 can be used with blocking peptide PEP-0418. The PA5-20284 immunogen is located within the first 50 amino acids of PKR. Predicted molecular ~ 62kD. In Western blot applications, this antibody has been observed to detect a band at: 68kD. Predicted species reactivity based on immunogen sequence: Mouse (86%).PKR is a 68 kDa protein and an IFN-induced serine/threonine protein kinase consisting of two N-terminal RNA-binding regulatory domains and a functional C-terminal serine/threonine protein kinase domain. Activated by dsRNA produced during viral infections, PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. Upon activation, PKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & maligcy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
|A peptide near the amino terminus of human PKR|
|Antigen affinity chromatography|
|Immunohistochemistry (Paraffin), Western Blot|
|PBS with 0.02% sodium azide|
|EIF2AK1, PKR, PRKR; P1/eIF-2A protein kinase; double stranded RNA activated protein kinase; eIF-2A protein kinase 2; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; p68 kinase; protein kinase, interferon-inducible double stranded RNA dependent; tyrosine-protein kinase EIF2AK2|
|Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
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