PLC-g1 (pY783) Mouse anti-Human, Alexa Fluor 647, Clone: 27/PLC, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 557883

Catalog No. BDB557883

Description

The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1-null mice exhibit retarded embryonic growth and lethality in midgestation. In addition, PDGF stimulation leads to phosphorylation of PLCg1 at Tyr 783 and activation of hydrolyzing activity. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus, regulation of PLCg activity by growth factors is involved in cell growth and transformation. The 27/PLC antibody recognizes PLCg1 phosphorylated at Y783. The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Mfr. No.612464) has been shown to react with human, mouse, and rat cell lysates by western blot.

Host Species: Mouse
Clone: 27
Isotype: IgG1
Species Reactivity: Human
Immunogen: Phosphorylated peptide including the tyrosine 783 of human PLC<special id='307'/>1

Flow Cytometry, Intracellular Staining

Specifications

• Antigen: PLC-g1 (pY783)
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 27/PLC
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Phosphorylated peptide including the tyrosine 783 of human PLCγ1
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1

For Research Use Only.