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pro-Caspase 3 Mouse anti-Human, Clone: 31A893, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA141163

Catalog No. 01-676-479


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Description

Description

Suggested positive control: HL-60 or staurosporine treated apoptotic HeLa.

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
Specifications

Specifications

pro-Caspase 3
Monoclonal
1.0 mg/mL
PBS with 0.05% sodium azide
P42574
A830040C14Rik, Apopain, CC3, CPP32, Caspase-3, Lice, Yama, mldy
Mouse
IgG
100 μg
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
836
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
31A893
Unconjugated
CASP3
Liquid
CASP3
Recombit full-length human Caspase-3.
Protein G
RUO
Antibody
Monoclonal
Human
Documents
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