Progesterone Receptor Mouse anti-Human, Clone: 4D8, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA123268

Catalog No. PIMA123268

Description

MA1-23268 detects Progesterone receptor in Human samples. MA1-23268 has been successfully used in Western Blot procedures. A suggested positive control for this product is MCF7. MA1-23268 immunogen corresponds to the region encoding amino acids 1-101 of the human progesterone receptor expressed in E. coli.. Store at 4°C short term. For extednded storage aliquot and store at -20°C or below, avoiding freeze/thaw cycles.

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintece of pregcy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintece of the endometrium, maintece of pregcy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

Specifications

• Antigen: Progesterone Receptor
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Gene: PGR
• Gene Alias: PR, NR3C3, PGR
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 5241
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Clone: 4D8
• Conjugate: Unconjugated
• Gene Accession No.: P06401
• Gene Symbols: PGR
• Immunogen: The region encoding amino acids 1-101 of the human progesterone receptor expressed in E. coli.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a