Properdin Mouse anti-Human, Clone: 10C5, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen HYB0390402

Catalog No. HYB0390402

Description

HYB 039-04-02 has specificity for human properdin. HYB 039-04-02 has been successfully used in ELISA and Western blotting procedures. HYB 039-04-02 reacts strongly with properdin isolated from human plasma when tested in sandwich ELISA using HYB 039-04-02 as capture and detection antibody, only very low reaction is seen with plasma from patients deficient in properdin. HYB 039-04-02 works equally well in ELISA with purified properdin coated directly onto the microtiter well. In Western blotting after SDS-PAGE HYB 039-04-02 reacts with properdin in both reduced (subunits of 25 kDa and 56 kDa) as well as unreduced forms (220 kDa). The HYB 039-04-02 immunogen is Properdin isolated from human plasma. Epitope specificity differs from that of HYB 039-06 but slightly overlap as determined by inhibition ELISA. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Properdin in plasma is a mixture of cyclic dimers, trimers and tetramers. The molecular weight of the glycosylated monomer is 53 kDa (3). Properdin is a regulator protein of the alternative complement pathway. It stabilizes the C3 convertase excerting its effect by binding to C3b in the C3bBb complex and thereby inhibiting cleavage of C3b by factor I and increasing the affinity for factor B. Serum concentration is approximately 25 µg/mL.

Specifications

• Antigen: Properdin
• Classification: Monoclonal
• Concentration: 1.11 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P27918
• Gene Alias: Factor P, PFC
• Host Species: Mouse
• Isotype: IgG1, kappa
• Quantity: 200 μL
• Storage Requirements: 4° C, store in dark
• Primary or Secondary: Primary
• Gene ID (Entrez): 5199

• Applications: ELISA, Western Blot
• Clone: 10C5
• Conjugate: Unconjugated
• Gene: CFP
• Format: Liquid
• Gene Symbols: CFP
• Immunogen: Properdin isolated from human plasma.
• Purification Method: Protein A
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human