PSMC5 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1970
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.PA1-970 detects proteasome 19S subunit S8 from human cells.PA1-970 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 45kDa protein representing proteasome 19S subunit S8 from HeLa cell lysate.PA1-970 antigen is recombinant proteasome 19S subunit S8.
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|S8, SUG-1, SUG1, TBP10, TRIP1, p45, p45/SUG|
|-20° C, Avoid Freeze/Thaw Cycles|
|Immunoprecipitation, Western Blot|
|Human recombit proteasome 19S subunit S8.|
|Ammonium sulfate precipitation|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok