DescriptionPTPN13/PTPL1 Polyclonal antibody specifically detects PTPN13/PTPL1 in Human samples. It is validated for Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin.
|A synthetic peptide corresponding to amino acids 1760-1775 (EPTRQENWTPLKNDLE) of human FAP-1 was used as immunogen; GenBank no. NP_542414.1. This peptide sequence is conserved in multiple isoforms of FAP-1. These FAP-1 isoforms are of varying amino acid (aa) lengths and include: (1) NP_542414.1 = 2485 aa, FAP-1 isoform 1. (2) BAD92141.1 = 2434 aa, FAP-1 isoform 2. (3) NP_542415.1 = 2294 aa, FAP-1 isoform 3. (4) NP_542416.1 = 2490 aa, FAP-1 isoform 4. (5) AAH39610.1 = 604 aa, FAP-1. (6) AAF63474.1 = 499 aa, FAP-1. For additional information on FAP-1 isoforms, users are encouraged to use the NCBI data bases such as AceView (http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/) or BLAST (http://www.ncbi.nlm.nih.gov/BLAST/).|
|Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)|
|Whole antisera with 0.02% Sodium Azide|
|FAP-1 typically migrates at ∼270 kDa on western blots. Thus for SDS-PAGE and western blot, the researcher should use techniques optimized for the transfer of proteins with high molecular weights. The actual molecular weight of FAP-1 observed depends on the isoform expression.|
For Research Use Only
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