RANBP2 Rabbit anti-Bovine, Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1082
PA1-082 detects Ran binding protein 2 (RanBP2) from human and bovine retina. PA1-082 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~360 kDa protein representing RanBP2 from bovine retinal extracts. Immunohistochemical staining of bovine retina with PA1-082 results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells. The PA1-082 immunogen is a synthetic peptide corresponding to residues M(1) R R S K A D V E R Y I A S V Q G S(18) of human RanBP2. This sequence is completely conserved between human and mouse. The PA1-082 immunizing peptide (Cat. No. PEP-137) is available for use in neutralization and control experiments.Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC, associated transport proteins and GTP. The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintece. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane.
Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
|Synthetic peptide corresponding to residues M(1) R R S K A D V E R Y I A S V Q G S(18) of human RanBP2.|
|Antigen affinity chromatography|
|Immunohistochemistry, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Ran Binding Protein 2, RBP2, Nuclear pore complex protein Nup358, Nucleoporin Nup358, 358 kDa nucleoporin, P270|
|-20° C, Avoid Freeze/Thaw Cycles|
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