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RANBP3 Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1084

Catalog No. PA1084

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PA1-084 detects Ran binding protein 3 (RanBP3) from human and rat cells. PA1-084 has been successfully used in Western blot and immunofluorescence procedures. By Western blot this antibody detects an ~60 kDa protein representing RanBP3 from HeLa cell extracts. Immunofluorescence staining of RanBP3 in NRK cells with PA1-084 results in diffuse nuclear staining. The PA1-084 immunogen is a synthetic peptide corresponding to residues M(1) A D L A N E E K P A L A(13) C of human RanBP3. The PA1-084 immunizing peptide (Cat. No. PEP-177) is available for use in neutralization and control experiments.

Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC, associated transport proteins and GTP. The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintece. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane. RanBP3 functions as a cofactor for CRM1-mediated nuclear export and stimulates export by enhancing the affinity of CRM1 for RanGTP and substrate.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly



Synthetic peptide corresponding to residues M(1) A D L A N E E K P A L A(13) C of human RanBP3.
Antigen affinity chromatography
Human, Rat
Immunocytochemistry, Immunofluorescence, Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
Ran Binding Protein 3, RANB3
200 μg
-20° C, Avoid Freeze/Thaw Cycles
501281, 8498
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