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Ref-1 Mouse, Unlabeled, Clone: 15, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 610816

Catalog No. BDB610816

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The AP-1 transcription factor consists of either Jun/Jun or Jun/Fos dimeric complexes. The transcriptional activity of Jun is enhanced by the phosphorylation of multiple sites in the activation domain or reduction of a conserved cysteine residue within the DNA binding domain. In contrast, oxidation of this cysteine residue inhibits AP-1 DNA binding. Ref-1 (redox factor 1) is a 36kDa ubiquitous nuclear protein that was originally purified from HeLa cells. It promotes AP-1 DNA binding by reducing this conserved cysteine residue. The DNA binding activity of many other transcription factors, such as NF-κB, Myb, and ATF/CREB family members, can be similarly induced by Ref-1. Ref-1 also functions in DNA repair like the class II apurinic/apyrimidinic (A/P) endonuclease. The A/P endonuclease repairs DNA lesions resulting from damage created by reactive oxygen radicals. It hydrolyzes DNA at the phosphate bond 5 ft. to abasic residues. Thus, Ref-1 is a multifuncitonal protein which is involved in DNA repair and gene expression via regulation of transcription factor DNA binding.

Immunofluorescence, Immunoprecipitation, Western Blotting



Canine, Human, Murine, Rat
Immunofluorescence, Western Blot
Affinity Purified
Human Ref-1 aa. 3-142
Cell Biology
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