Ref-1 Mouse, Unlabeled, Clone: 15, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 610816
The AP-1 transcription factor consists of either Jun/Jun or Jun/Fos dimeric complexes. The transcriptional activity of Jun is enhanced by the phosphorylation of multiple sites in the activation domain or reduction of a conserved cysteine residue within the DNA binding domain. In contrast, oxidation of this cysteine residue inhibits AP-1 DNA binding. Ref-1 (redox factor 1) is a 36kDa ubiquitous nuclear protein that was originally purified from HeLa cells. It promotes AP-1 DNA binding by reducing this conserved cysteine residue. The DNA binding activity of many other transcription factors, such as NF-κB, Myb, and ATF/CREB family members, can be similarly induced by Ref-1. Ref-1 also functions in DNA repair like the class II apurinic/apyrimidinic (A/P) endonuclease. The A/P endonuclease repairs DNA lesions resulting from damage created by reactive oxygen radicals. It hydrolyzes DNA at the phosphate bond 5 ft. to abasic residues. Thus, Ref-1 is a multifuncitonal protein which is involved in DNA repair and gene expression via regulation of transcription factor DNA binding.
Immunofluorescence, Immunoprecipitation, Western Blotting
|Canine, Human, Murine, Rat|
|Immunofluorescence, Western Blot|
|Human Ref-1 aa. 3-142|
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