anti-Retinoic Acid Receptor alpha, Polyclonal, Invitrogen
Applications: IP, WB
Manufacturer: Thermo Scientific PA1810
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: α, β and γ. The corresponding gene for the the retinoic acid receptor α is NR1B1.
Host Species: Mouse
Species Reactivity: Rat, Mouse, Human, Non-human primate
Immunogen: Synthetic peptide corresponding to residues C(444) S P S L S P S S H R S S P A T Q S P(462) of mouse RAR α
MA1-810 detects retinoic acid receptor (RAR) α from human, rat and mouse tissues and overexpressed cells. This antibody does not detect any of the RAR β or γ forms. MA1-810 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single 45kDa protein representing RAR α in transfected COS cells. Due to the extremely low levels of RAR expressed in native tissues, it is recommended that sensitive detection systems, such as enhanced chemiluminescence, be used. The apparent molecular weight of RAR α from transfected COS cells is 45kDa. In rat tissues, such as skin and testis, doublets in the 50kDa area are seen. RAR α has been identified with this antibody in human dermal and neonatal fibroblast cell lines, as well as mouse F9 and human SH-SY5Y cells; however, RAR α from human leukocytes and HUT102 cells were not identified by MA1-810 suggesting that there may be structural variation of RAR α between some cell types. The MA1-810 immunogen is a synthetic peptide corresponding to residues C(444) S P S L S P S S H R S S P A T Q S P(462) of mouse RAR α. This peptide (Cat. No. PEP-004) is available for use in neutralization and control experiments.
Immunoprecipitation, Western Blotting
Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
|Retinoic Acid Receptor alpha|
|Synthetic Peptide: C S(444) P S L S P S S H R S S P A T Q S P(462)|
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