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ROCK-I Mouse, Unlabeled, Clone: 46, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 611137

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Catalog No. BDB611137


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Description

Description

ROCK-I is a Rho-associated serine/threonine kinase isozyme that mediates RhoA-induced assembly of focal adhesions and actin stress fibers. It contains an N-terminal kinase domain, a central 600 amino acid long coiled-coil region, a C-terminal pleckstrin homology region (PH) and a Cys-rich zinc finger motif. The ROCK-I kinase domain is approximately 90% identical to that of ROCK-II. ROCK-I binds GTP-bound Rho through a Rho-binding domain (RBD). As a result, the kinase activity of ROCK-I is moderately stimulated. The ROCK isozymes regulate cell contractility through phosphorylation of the myosin light chain. This effect results from either the inhibition of the myosin phosphatase or by direct phosphorylation of the myosin light chain, thus bypassing the myosin light chain kinase. In addition, ROCK-I activates the ubiquitously expressed Na-H exchanger (NHE1) via a number of mechanisms including RhoA. NHE1 may mediate ROCK-I-induced changes in the actin cytoskeleton. Therefore, ROCK-I plays an important role in the regulation of focal adhesion and stress fiber formation.

Host Species: Mouse
Clone: 46
Isotype: IgG1
Species Reactivity: Mouse
Immunogen: Mouse ROCK-I aa. 906-1012
Formula Weight [Chemical]: 160kDa

Immunofluorescence, Western Blotting

Specifications

Specifications

ROCK-I
46
Unconjugated
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Murine
IgG1
RUO
Store undiluted at -20°C.
Monoclonal
Western Blot
250μg/mL
ROKβ
Affinity Purified
Mouse ROCK-I aa. 906-1012
150μg
Cell Biology
Primary
Human, Murine, Rat
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