S100A1 Rabbit anti-Bovine, Human, Mouse, Porcine, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1932
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein. PA1-932 has successfully been used in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane. The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells.
|PBS with 15mg/mL BSA and 0.05% sodium azide|
|P02639, P23297, P56565, P35467|
|-20° C, Avoid Freeze/Thaw Cycles|
|106504218, 20193, 295214, 528735, 6271|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|Purified S100-alpha protein from human pectoral muscle cells.|
|Antigen affinity chromatography|
|Bovine, Human, Mouse, Porcine, Rat|
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