SARS Spike Protein Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 15 publications

Supplier:  Novus Biologicals NB10056578SS

Catalog No. NB10056578T

Description

SARS Spike Protein Polyclonal specifically detects SARS Spike Protein in SARS-CoV, SARS-CoV-2 samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: Sars Spike Protein
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% Sodium Azide
• Host Species: Rabbit
• Purification Method: Immunogen affinity purified
• Regulatory Status: RUO
• Gene ID (Entrez): 1489668
• Target Species: SARS-CoV, SARS-CoV-2
• Form: Purified

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Concentration: 1.0 mg/ml
• Dilution: Western Blot 0.05 - 1.0 ug/mL, Simple Western 1:40 - 1:160, Immunohistochemistry reported in scientific literature (PMID 34357881), Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 16081529)
• Gene Alias: COVID-19 Spike, E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARSCOV2 Spike protein, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2
• Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-GNCDVVIGIVNNTVYDP) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. YP_009724390)
• Quantity: 0.025 mg
• Primary or Secondary: Primary
• Test Specificity: Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

For Research Use Only