AANAT Rabbit anti-Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1762
PA1-762 has successfully been used to detect serotonin N-AT in both WB and IF applications. PA1-762 detects serotonin N-acetyltransferase (AA-NAT) from rat tissues. PA1-762 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. PA1-762 immunizing phosphopeptide (Cat. No. PEP-066) corresponds to carboxy-terminal amino acid residues 198-208 of rat AA-NAT. Total anti-carboxy-terminal AA-NAT-specific IgG (Cat. No. PA1-762) was affinity purified on resin-coupled non-phosphopeptide (Cat. No. PEP-065). Phospho-specific carboxy-terminal IgG (Cat. No. PA1-763) was subsequently isolated on resin-coupled phosphopeptide (Cat. No. PEP-066). This sequence is completely conserved between rat and mouse AA-NAT protein.Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
|Synthetic Peptide: C(195) H T F L R R N S G C(205)|
|Antigen affinity chromatography|
|Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|-20° C, Avoid Freeze/Thaw Cycles|
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