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SHC Mouse, Unlabeled, Clone: 30, BD
Mouse Monoclonal Antibody
Supplier: BD Biosciences 610878
Description
The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66kDa), each contain a C-terminal SH2 domain and an N-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway. To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs.
Phosphorylated SHC forms a complex with the adaptor protein GRB2. Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane. Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange. Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases. The SHC proteins are mediators of signals that are essential
for cell metabolism, growth, and differentiation.
Immunofluorescence, Western Blotting
Specifications
SHC | |
Monoclonal | |
250μg/mL | |
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. | |
Human SHC aa. 359-473 | |
50 μg | |
Cell Biology | |
Canine, Chicken, Human, Mouse, Rat | |
IgG1 |
Western Blot | |
30 | |
Unconjugated | |
Mouse | |
Affinity Purified | |
RUO | |
Primary | |
Store undiluted at -20°C. |
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For Research Use Only.