SKA2 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA520818
A suggested positive control is NIH-3T3 cell lysate. PA5-20818 can be used with blocking peptide PEP-0932.Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintece of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.
|A 15 amino acid peptide from near the amino terminus of human SKA2.|
|Antigen affinity chromatography|
|PBS with 0.02% sodium azide|
|FAM33A; family with sequence similarity 33, member A; spindle and KT (kinetochore) associated 2; spindle and kinetochore-associated protein 2|
|Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
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