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SMAD3 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA138613

Catalog No. 01-673-737

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Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is normal breast or breast carcinoma.

Smad proteins mediate signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily. Biological activities mediated by smads include cell growth and morphogenesis, development, and immune response. A reduction in Smad2/3 signaling enhances tumorigenesis, but also suppresses metastasis of breast cancer cell lines.


PBS with 1% BSA and 0.1% sodium azide; pH 7.5
Q8BUN5, P84025, P84022
HSPC193, HsT17436, JV15-2, LDS1C, MADH3, MAD homolog 3, MAD, mothers against decapentaplegic homolog 3, SMA- and MAD-related protein 3, SMAD, mothers against DPP homolog 3, hMAD-3, hSMAD3, mad homolog JV15-2, mad protein homolog, mad3, mothers against DPP homolog 3, mothers against decapentaplegic homolog
1 mL
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
17127, 25631, 4088
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Synthetic peptide corresponding to internal region of human SMAD3.
Antigen affinity chromatography
Human, Mouse, Rat
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