Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

SREBP1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1337

Catalog No. PA1337


Add to cart

Description

Description

PA1-337 detects SREBP 1 in mouse and rat samples. PA1-337 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished. The PA1-337 immunogen is a synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1. This sequence is identical in human and rat. PA1-337 immunizing peptide (Cat. No. PEP-274) is available for use in neutralization and control experiments.

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.
Specifications

Specifications

SREBP1
Polyclonal
Unconjugated
Srebf1
Liquid
SREBF1
Synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
P56720, P36956, Q9WTN3
Sterol regulatory element binding protein 1, SREBP-1, Sterol regulatory element-binding transcription factor 1, nt-SREBP1
Rabbit
IgG
100 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
78968, 20787, 6720
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit