SFTPA1 Mouse anti-Human, Biotin, Clone: 6F10, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen HYB23804B01
HYB 238-04B-01 detects Surfactant protein A in human samples . HYB 238-04B-01 has been successfully used in ELISA procedures. In Western blotting after SDS-PAGE, HYB 238-04B-01 reacts with reduced forms of SP-A and weaker with non-reduced Forms. In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04B-01 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. The HYB 238-04B-01 immunogen is native SP-A purified from bronchioaveolar lavage from proteinosis patients. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mLSurfactant protein A (SP-A) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular ft.head ft. regions linked by triple-helical, collagen-like, strands. This group also includes SP-D and the serum proteins man-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45 ng/mL in healthy individuals.
|PBS with 15mM sodium azide; pH 7.4|
|Pulmonary surfactant-associated protein A, SP-A|
|4° C, store in dark|
|Native SP-A purified from bronchioaveolar lavage from proteinosis patients.|