Tet Repressor Rabbit anti-Bacteria, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA129677
PA1-29677 detects Tet Repressor from many samples. This antibody works in any species where the TetR system is being used to regulate gene ezpression in mammalian cells. PA1-29677 has been successfully used in Western blot and ELISA applications. Not suitable for immunofluorescence. The PA1-29677 immunogen is residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes. Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below, avoiding multiple freeze-thaw cycles.The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determits in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|ELISA, Western Blot|
|PBS with 20% glycerol and 0.025% Proclin 300|
|TetR, Tetracycline receptor|
|Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.|
|Antigen affinity chromatography|
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