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TEV Cleavage Site Rabbit anti-Tag, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1119

Catalog No. PIPA1119


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Description

Description

PA1-119 has been successfully used in Western blot applications. The PA1-119 immunogen is a peptide representing the cleavage site recognized by TEV enzyme.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
Specifications

Specifications

TEV Cleavage Site
Polyclonal
Unconjugated
Liquid
Rabbit
IgG
50 μg
4° C
Primary
Tag
Western Blot
0.5 mg/mL
BBS with no preservative; pH 8.4
Tobacco Etch Virus
Peptide representing the cleavage site recognized by TEV enzyme
Antigen affinity chromatography
RUO
Antibody
Polyclonal
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