TrxR1 Mouse anti-Human, Clone: 5A5, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen LFMA0020
A suggested positive control for this product is HeLa cells.The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxidoreductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence (-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena.
|Purified, recombit, human thioredoxin reductase 1 protein expressed in E. coli.|
|Ammonium sulfate precipitation|
|HEPES with 0.01% BSA, 0.15M NaCl, 50% glycerol and 0.03% sodium azide|
|-20° C, Avoid Freeze/Thaw Cycles|
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