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TIN2 Mouse anti-Human, Mouse, Clone: 59B388.1, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA141038

Catalog No. 01-676-448


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Description

Description

TRF1 is a negative regulator of telomere length maintenance (3). Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (2,3). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening (1). Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenousTRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.

Suggested positive control: 293 whole cell lysate, antigen standard for TINF2 (transient overexpression lysate).
Specifications

Specifications

TIN2
Monoclonal
0.5 mg/mL
PBS with 0.05% BSA and 0.05% sodium azide
Q9BSI4, Q9QXG9
Tin-2
Mouse
IgG1
100 μg
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
26277, 28113
Gel Shift, Immunocytochemistry, Immunofluorescence, Western Blot
59B388.1
Unconjugated
TINF2
Liquid
TINF2
Synthetic peptide corresponding to residues 44-58 of human Tin2.
Protein G
RUO
Antibody
Monoclonal
Human, Mouse
Documents
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