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TNF alpha Mouse anti-Rat, Clone: 45418, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA523741

Catalog No. PIMA523741


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Description

Description

In ELISAs, this antibody shows less than 3% cross-reactivity with Recombinant mouse (rm) TNF- alpha and less than 0.2% cross-reactivity with rhTNF- alpha, rpTNF- alpha, and rhTNF- beta. In Western blots, this antibody shows 100% cross-reactivity with rmTNF- alpha and no cross-reactivity with rfeTNF- alpha. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Specifications

Specifications

TNF alpha
Monoclonal
0.5 mg/mL
PBS with 5% trehalose and No Preservative
P16599
APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, cachectin, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha, TNFA
Mouse
IgG1
500 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
24835
ELISA, Neutralization, Western Blot
45418
Unconjugated
Tnf
Lyophilized
TNF
E. coli-derived recombit rat TNF-alpha
Protein A/G
RUO
Antibody
Monoclonal
Rat
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