TNF-alpha Antibody - Azide Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 18 publications

Supplier:  Novus Biologicals NB600587

Catalog No. NB600587

Description

TNF-alpha Polyclonal specifically detects TNF-alpha in Human, Mouse, Rat, Chicken, Primate samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: TNF-alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Antiserum with No Preservative
• Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Host Species: Rabbit
• Molecular Weight of Antigen: 25.64 kDa
• Quantity: 0.2 mL
• Research Discipline: Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Gene ID (Entrez): 7124
• Target Species: Human, Mouse, Rat, Primate
• Form: Antisera

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Concentration: 75 mg/mL
• Dilution: Western Blot 1:500-1:2000, ELISA 1:1000-1:5000, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100-1:500
• Gene Accession No.: P01375, P01375, P01375, P01375
• Gene Symbols: TNF
• Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human TNF-alpha produced in E.coli. (Uniprot: P01375)
• Purification Method: Whole Antiserum
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

For Research Use Only