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TNF alpha Mouse anti-Porcine, Clone: 6E10, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MP391

Catalog No. PIMP391


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Description

Description

MP391 detects natural and recombit TNF alpha, with a predicted molecular weight of approximately 25 kDa. This is a carrier-free low endotoxin formulation, <1.0 EU/mg. This product can be used as a coating antibody in a sandwich ELISA (100 µg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product No. M302B). For Western blot, this antibody has been successfully used in conjunction with 0.02 µg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product No. 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product No. N100), and recombit Porcine TNFa (Product No. RPTNFAI), followed by visualization using SuperSignal™ West Pico Chemiluminescent Substrate (Product No. 34080). This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Specifications

Specifications

TNF alpha
Monoclonal
1 mg/mL
PBS with no preservative
P23563
TNF-a; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor-necrosis factor
Mouse
IgG1
500 μg
-20°C
Primary
397086
ELISA, Western Blot
6E10
Unconjugated
TNF
Liquid
TNF
Recombit porcine TNF alpha (Baculovirus-derived)
Protein A
RUO
Antibody
Monoclonal
Porcine
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