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anti-TRAF-2, Polyclonal, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 1 publication

Manufacturer:  Novus Biologicals NB10056172

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Catalog No. NB10056172

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TRAF-2 Polyclonal specifically detects TRAF-2 in Human, Mouse, Rat samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.


Whole antisera with 0.05% Sodium Azide
E3 ubiquitin-protein ligase TRAF2, EC 6.3.2.-, MGC:45012, TNF receptor-associated factor 2, TRAP3TRAP, tumor necrosis factor type 2 receptor associated protein 3, Tumor necrosis factor type 2 receptor-associated protein 3
0.05 ml
Apoptosis, Cancer, Cytokine Research, Zinc Finger
Human, Mouse, Rat
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Western Blot 1/1000 - 1/200, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1/1000 - 1/500, Immunohistochemistry-Frozen Use at an assay dependent dilution
A synthetic peptide corresponding to amino acids 87-106 (SSSAFPDNAARREVESLPAV) of human TRAF2 was used as immunogen, GenBank no. NP_066961.2. This peptide sequence is 100% conserved in mouse and rat TRAF2. The peptide immunogen sequence is 100% conserved in multiple human TRAF2 GenBank accession numbers. These TRAF2 accession numbers are of varying amino acid (aa) lengths and include: 1. NP_066961.2 = 501 aa. 2. CAI12705.1 =121 aa. 3. CAI12704.1 = 422 aa. 4. CAI15104.1 = 193 aa. 5. BAD96681.1 = 205 aa.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.
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