Tubby Mouse anti-Mouse, Unlabeled, Clone: 40, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 612106
Tubby is an autosomal recessive syndrome characterized by maturity-obesity, insulin resistance, infertility, and cochlear and retinal degeneration. Tubby protein (Tub) is highly expressed in brain, especially hypothalamus where body weight regulation is controlled. The sequence of normal tubby includes putative tyrosine phosphorylation sites for SH-2 protein binding and a nuclear localization signal (NLS), while mutant tubby contains a 24 intron amino-acid insert substituted for 44 C-terminal amino acids. In PC12 cells, insulin induces tyrosine phosphorylation. In vitro, tubby is phosphorylated by insulin receptor kinase, Abl, JAK2, and upon phosphorylation tubby associates with the SH2 domains of Abl, Lck, phospholipase Cγ. The C-terminal region of tubby binds to phosphatidylinositol 4,5-bis-phosphate, which facilitates localization to the plasma membrane. Receptor-mediated activation of Gαq releases tubby from the plasma membrane through the action of phospholipase C-B. This allows translocation of tubby to the nucleus where it may regulate transcription. Thus, tubby is a transcription regulator activated by the G-protein phospholipid signaling pathway.
|In vitro Assay, Western Blot|
|Mouse Tubby aa. 102-205|
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