UbcH6 Mouse, Unlabeled, Clone: 42, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611218

Catalog No. BDB611218

Description

Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an ε-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome. Ubiquitination requires ubiquitin-activating enzyme E1, ubiquitin conjugating enzymes E2, and ubiquitin ligases E3. The direction of ubiquitin transfer is from E1 to E2 and from E2 to E3. E6-AP is a human E3 which, in complex with the E6 protein of human papillomaviruses, induces the ubiquitination and degradation of p53. UbcH6 is a human homolog of the S. cerevisiae ubiquitin-conjugating enzyme (Ubc), UBC4, which interacts with E6-AP. UbcH6 also interacts with E6-AP. However, it has little effect on E6-AP-dependent ubiquitination. The precise physiological function of UbcH6 remains to be determined.

Host Species: Mouse
Clone: 42
Isotype: IgG1
Species Reactivity: Human
Immunogen: Human UbcH6 aa. 46-58
Formula Weight [Chemical]: 21kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: UbcH6
• Classification: Monoclonal
• Concentration: 250μg/mL
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Isotype: IgG1

• Applications: Immunofluorescence, Western Blot
• Clone: 42
• Conjugate: Unconjugated
• Immunogen: Human UbcH6 aa. 46-58
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Human, Mouse, Rat