UCP3 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA124895
PA1-24895 detects UCP3 from human, mouse, and rat samples. The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids respectively. This antibody exhibits no cross reactivity with UCP1 or UCP2. PA1-24895 has been successfully used in Western blot and immunohistochemistry (paraffin) procedures. The PA1-24895 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 254-267 of human UCP3 with an N-terminal added lysine. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.The uncoupling proteins (UCPs) are found in the inner mitochodrial membranes and belong to a family of mitochondrial anion transport proteins that include phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissues of mammals. UCP 1 allows proton re-entry into mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is though that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by th e respiratory chaun in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predomitly in brown adipose tissue, skeletal muscle, at lower levels in the heart and smooth muscle, and is absent in the liver and kidneys.
|Synthetic peptide, conjugated to KLH, corresponding to residues 254-267 of human UCP3 with an N-terminal added lysine.|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunohistochemistry (Paraffin), Western Blot|
|0.01M PBS with 1% BSA and 15mM sodium azide; pH 7.4|
|P55916, P56501, P56499|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|22229, 25708, 7352|
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