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anti-UNG, Polyclonal, Novus Biologicals™

Rabbit Polyclonal Antibody

Manufacturer:  Novus Biologicals NB6001031

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Catalog No. NB6001031


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Description

Description

UNG Polyclonal antibody specifically detects UNG in Human, Mouse, Rat, Rabbit samples. It is validated for Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
Specifications

Specifications

UNG
1.0 mg/ml
Western Blot 2 ug/ml, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 10 ug/ml
NP_550433
DGUEC 3.2.2, HIGM4EC 3.2.2.27, UDGDKFZp781L1143, UNG15, UNG1uracil-DNA glycosylase 1, uracil-DNA glycosylase 2, UNG2, uracil-DNA glycosylase
Rabbit
IgG
0.1 mg
Base Excision Repair, DNA Repair, Stem Cell Markers
Primary
7374
Human, Mouse, Rat, Rabbit
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Unconjugated
PBS with 0.05% Sodium Azide
Purified
UNG
This antibody was generated by immunizing rabbit with a synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP 003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates.
Protein G purified
RUO
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Polyclonal
The synthetic peptide sequence used for immunogen is 100% conserved between UNG1 and UNG2. Thus the antibody recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kDa, 27.5 kDa, 31 kDa, and 38 kDa forms have been described (Bharati et al., 1998; Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this antibody has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).
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